![]() ![]() These data suggest that peptides obtained from enzymatic hair hydrolysis may be useful for future applications on pharmaceutical and cosmetic formulations. subtilis strain grown in a medium containing human hair. This is the first report describing the production and partial characterization of keratinases by a B. subtilis AMR was able to hydrolyse human hair producing serine peptidases with keratinase and gelatinase activity as well as hair keratin peptides. subtilis AMR revealed fragments in a range of 800-2600 Da. MALDI-TOF analysis of culture supernatant containing peptides produced during enzymatic hydrolysis of hair by B. ![]() Scanning electron microscopy showed complete degradation of the hair cuticle after exposure to B. Keratinases were optimally active at 50 degrees C and pH 9.0 and was fully inhibited by the serine proteinase inhibitor (PMSF). Properties (Bacillus subtilis) Catalase: Positive (+ve) Citrate: Positive (+ve) Flagella: Flagellated: Gas: Negative (-ve) Gelatin Hydrolysis: Positive (+ve) Gram Staining: Gram Positive (+ve) Indole: Negative (-ve) Motility: Positive (+ve) MR (Methyl Red) Negative (-ve) Nitrate Reduction: Positive (+ve) Oxidase: Variable: Pigment: Negative. 54, 80 and 100 kDa and gelatin-degrading bands at c. ![]() Zymography showed keratin-degrading peptidases migrating at c. Keratinase yield was not correlated with increase in biomass. subtilis AMR presented high keratinase production on the 8th day of fermentation in hair medium (HM) supplemented with 0.01% yeast extract (HMY) at pH 8.0. The supplementation of hair medium (HM) with 0.01% yeast extract increased the keratinolytic activity 4.2-fold. The isolate was grown in medium containing human hair as the sole source of carbon and nitrogen. Negative gelatin hydrolysis exhibited by Escherichia coli (B) is indicated by the absence of a clearing zone around the colony. What is the reference bacterium for the gelatin hydrolysis test B. Positive gelatin hydrolysis exhibited by Bacillus subtilis (A) is indicated by the clear zone around the colony after the addition of saturated ammonium sulfate. The effective hydrolysis of gelatin and skim milk proteins indicated that. What is a positive result of the Casein hydrolysis test. The Bacillus subtilis AMR was identified using biochemical tests and by analysis of 16S rDNA sequence. test, Indole test, H2S production, Lysine decarboxyl- ation, Arginine. Minimum inhibition zone was recorded by BS-6 which is 15.20 mm.To determine the ability of a novel Bacillus subtilis AMR isolated from poultry waste to hydrolyse human hair producing peptidases including keratinases and hair keratin peptides. Also done and BS-5 recorded maximum inhibition zone which is 16.60 mm followed by BS-2 which is 16.20 mm. In this investigation antagonistic study of Bacillus subtilis against Xanthomonas axonopodis pv. All the isolates were found positive for catalase test (except BS-4), positive for gelatine liquefaction, negative for KOH test, positive for acid and gas production, positive for H 2S test (except BS-1 and BS-6), positive for starch hydrolysis (except BS-2, BS-5, BS-7), negative for IAA production, positive for phosphate solubilization (except BS-4). Prepared gelatine agar and distributed in three boiling tubes (two for two bacteria and one for control). All the isolates shows maximum growth at 30 and 35✬ temperature and grew well in 6 to 8 pH range. The bacteria found to be Gram positive and rod shaped. Gram reaction, cell shape, colony, H 2S production, gelatin liquefaction, starch hydrolysis, catalase test, KOH test, acid and gas production, phosphate solubilization and IAA production test were carried out. The isolates were characterized by standard biochemical and physiological characters viz. ![]() Out of 15 samples only 7 samples yielded Bacillus subtilis on the King’s B medium. Gram reaction, cell shape, colony, H2S production, gelatin liquefaction, starch hydrolysis, catalase test, KOH test, acid and gas production, phosphate. Fifteen isolates of Bacillus subtilis were isolated from rhizospheric soil of different crops. Biochemical and physiological characterizations of Bacillus subtilisĪuthor(s): CA Sarode, SB Bramhankar, SA Kakad, AA Labhasetwar, SS Bhure, SS Isokar, GT Dinkwar, SV Bambal, and DG TathodĪbstract: An investigation entitled “Biochemical and physiological characterizations of Bacillus subtilis” was undertaken at Plant Pathology Section, College of Agriculture, Nagpur during the year s2017-18. ![]()
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